Active Uptake of Ca++ and Ca++-Activated Mg++ ATPase in Red Cell Membrane Fragments
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Active Uptake of Ca++ and Ca++-Activated Mg++ ATPase in Red Cell Membrane Fragments
Isolated human red blood cell membrane fragments (RBCMF) were found to take up Ca(++) in the presence of ATP.(1) This ATP-dependent Ca(++) uptake by RBCMF appears to be the manifestation of an active Ca(++) transport mechanism in the red cell membrane reported previously (Schatzmann, 1966; Lee and Shin, 1969). The influences of altering experimental conditions on Ca(++)-stimulated Mg(++) ATPase...
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The membrane-bound, solubilized, and trypsin-treated forms of Mg, Ca-ATPase from E. coli are inhibited by ruthenium red [RR]. The inhibition is noncompetitive and is reduced at higher substrate concentrations. n-Butanol-extracted ATPase is not inhibited by ruthenium red and is not activated by KCl.
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ژورنال
عنوان ژورنال: Journal of General Physiology
سال: 1971
ISSN: 1540-7748,0022-1295
DOI: 10.1085/jgp.57.2.202